Free Access
Reprod. Nutr. Dev.
Volume 43, Number 1, January-February 2003
Page(s) 41 - 55


  1. Blaxter KL, Czerkawski J. Modifications of the methane production of the sheep by supplementation of its diet. J Sci Food Agric 1966, 17: 417-421.
  2. Machmüller A, Kreuzer M. Methane suppression by coconut oil and associated effects on nutrient and energy balance in sheep. Can J Anim Sci 1999, 79: 65-72.
  3. Vogels GD, Hoppe WF, Stumm CK. Association of methanogenic bacteria with rumen ciliates. Appl Environ Microbiol 1980, 40: 608-612.
  4. Finlay BJ, Esteban G, Clarke KJ, Williams AG, Embley TM, Hirt RP. Some rumen ciliates have endosymbiotic methanogens. FEMS Microbiol Lett 1994, 117: 157-162.
  5. Stumm CK, Gijzen HJ, Vogels GD. Association of methanogenic bacteria with ovine rumen ciliates. Br J Nutr 1982, 47: 95-98.
  6. Newbold CJ, Lassalas B, Jouany JP. The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. Lett Appl Microbiol 1995, 21: 230-234.
  7. Dohme F, Machmüller A, Estermann BL, Pfister P, Wasserfallen A, Kreuzer M. The role of the rumen ciliate protozoa for methane suppression caused by coconut oil. Lett Appl Microbiol 1999, 29: 187-192.
  8. Sharp R, Ziemer CJ, Stern MD, Stahl DA. Taxon-specific associations between protozoal and methanogen populations in the rumen and a model rumen system. FEMS Microbiol Ecol 1998, 26: 71-78.
  9. Kreuzer M, Kirchgessner M. Die Bedeutung der Protozoen im Pansen von Hammeln für Verdaulichkeit, N-Bilanz und N-Fraktionen in Kot und Harn bei unterschiedlichen Gehalten und Arten von Stärke in der Ration. J Anim Physiol Anim Nutr 1986, 56: 217-231.
  10. Dohme F, Machmüller A, Wasserfallen A, Kreuzer M. Comparative efficiency of various fats rich in medium-chain fatty acids to suppress ruminal methanogenesis as measured with RUSITEC. Can J Anim Sci 2000, 80: 473-482.
  11. RAP. Fütterungsempfehlungen und Nährwerttabellen für Wiederkäuer. Landwirtschaftliche Lehrmittelzentrale, Zollikofen. 1999.
  12. Naumann K, Bassler R. Methodenbuch, Band III, Die chemische Untersuchung von Futtermitteln. VDLUFA-Verlag, Darmstadt. 1997.
  13. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991, 74: 3583-3597.
  14. Hoffmann L, Klein M. Die Abhängigkeit der Harnenergie vom Kohlenstoff- und Stick- stoffgehalt im Harn bei Rindern, Schafen, Schweinen und Ratten. Arch Tierernähr 1980, 30: 743-750.
  15. Tangerman A, Nagengast FM. A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method. Anal Biochem 1996, 236: 1-8.
  16. Stahl DA, Amann RI, Poulson LK, Raskin L, Capman WC. Use of fluorescent probes for determinative microscopy of methanogenic archaea. In: Sowers KR, Schreier HJ (Eds), Archaea, a Laboratory Manual, Methanogens. Cold Spring Harbor Laboratory Press, New York. 1995, p 111-121.
  17. Soliva CR, Hindrichsen IK, Gerig C, Meile L, Kreuzer M, Machmüller A. Effect of a stepwise increase of medium-chain fatty acid supply on ruminal methanogenesis in vitro. In: Takahashi J, Young BA (Eds), Greenhouse Gases and Animal Agriculture, Elsevier Science, Amsterdam, 2002. p 161-165.
  18. Lin C, Raskin L, Stahl DA. Microbial community structure in gastrointestinal tracts of domestic animal: comparative analyses using rRNA-targeted oligonucleotide probes. FEMS Microbiol Ecol 1997, 22: 281-294.
  19. Brouwer E. Report of sub-committee on constants and factors. In: Blaxter KL (Ed), Energy Metabolism. Academic Press, London, 1965. p 441-443.
  20. AFRC. Energy and protein requirements of ruminants. CAB International, Wallingford. 1993.
  21. Veira DM, Ivan M. Rumen ciliate protozoa: Effects on digestion in the stomach of sheep. J Dairy Sci 1983, 66: 1015-1022.
  22. Jouany JP, Demeyer DI, Grain J. Effect of defaunating the rumen. Anim Feed Sci Technol 1988, 21: 229-265.
  23. Itabashi H, Kobayashi T, Matsumoto M. The effects of rumen ciliate protozoa on energy metabolism and some constituents in rumen fluid and blood plasma of goats. Jpn J Zootech Sci 1984, 55: 248-256.
  24. Demeyer DI, Van Nevel CJ, Van de Voorde G. The effect of defaunation on the growth of lambs fed three urea containing diets. Arch Tierernähr 1982, 32: 595-604.
  25. Mathison GW, Okine EK, McAllister TA, Dong Y, Galbraith J, Dmytruk OIN. Reducing methane emissions from ruminant animals. J Appl Anim Res 1998, 14: 1-28.
  26. Moss AR, Jouany JP, Newbold J. Methane production by ruminants: its contribution to global warming. Ann Zootech 2000, 49: 231-253.
  27. Van Nevel C, Demeyer D. Feed additives and other interventions for decreasing methane emissions. In: Wallace RJ, Chesson A (Eds), Biotechnology in Animal Feeds and Animal Feeding. VCH, Weinheim, 1995. p 329-349.
  28. Hegarty RS. Reducing rumen methane emissions through elimination of rumen protozoa. Aust J Agric Res 1999, 50: 1321-1327.
  29. Chandramoni SB, Jadhao CM, Tiwari N, Haque M, Khan MY. Energy metabolism and methane production in faunated and defaunated sheep fed two diets with same concentrate to roughage ratio (70:30) but varying in composition. Asian-Austral J Anim Sci 2001, 14: 1238-1244.
  30. Eadie JM, Gill JC. The effect of the absence of rumen ciliate protozoa on growing lambs fed on a roughage-concentrate diet. Br J Nutr 1971, 26: 155-167.
  31. Kreuzer M, Kirchgessner M, Müller HL. Effect of defaunation on the loss of energy in wethers fed different quantities of cellulose and normal or steamflaked maize starch. Anim Feed Sci Technol 1986, 16: 233-241.
  32. Vermorel M, Jouany JP. Effects of rumen protozoa on energy utilization by wethers of two diets based on ammonia-treated straw supplemented or not with maize. Asian-Austral J Anim Sci 1989, 2: 475-476.
  33. Whitelaw FG, Eadie JM, Bruce LA, Shand WJ. Methane formation in faunated and ciliate-free cattle and its relationship with rumen volatile fatty acid productions. Br J Nutr 1984, 52: 261-275.
  34. Ushida K, Tokura M, Takenaka A, Itabashi H. Ciliate protozoa and ruminal methanogenesis. In: Onodera R, Itabashi H, Ushida K, Yano H, Sasaki Y (Eds), Rumen Microbes and Digestive Physiology in Ruminants. Japan Scientific Societies Press, Tokyo, 1997. p 209-220.
  35. Ushida K, Miyazaki A, Kawashima R. Effect of defaunation on ruminal gas and VFA production in vitro. Jpn J Zootech Sci 1986, 57: 71-77.
  36. Morvan B, Bonnemoy F, Fonty G, Gouet P. Quantitative determination of H2-utilizing acetogenic and sulfate-reducing bacteria and methanogenic archaea from digestive tract of different mammals. Curr Microbiol 1996, 32: 129-133.
  37. Machmüller A, Ossowski DA, Kreuzer M. Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Anim Feed Sci Technol 2000, 85: 41-60.
  38. Murray RM, Bryant AM, Leng RA. Methane production in the rumen and lower gut of sheep given lucerne chaff: effect of level of intake. Br J Nutr 1978, 39: 337-345.
  39. Machmüller A, Soliva CR, Kreuzer M. In vitro ruminal methane suppression by lauric acid as influenced by dietary calcium. Can J Anim Sci 2002, 82: 233-239.
  40. Doré J, Mackie RI. Microbial diversity in gut ecosystem. Reprod Nutr Dev 1997, 5: 15-20.
  41. Leedle JAZ, Greening RC. Methanogenic and acidogenic bacteria in the bovine rumen: postprandial changes after feeding high- or low-forage diets once daily. Appl Environ Microbiol 1988, 54: 502-506.
  42. Sutton JD, Knight R, McAllan AB, Smith RH. Digestion and synthesis in the rumen of sheep given diets supplemented with free and protected oils. Br J Nutr 1983, 49: 419-432.
  43. Broudiscou L, Van Nevel CJ, Demeyer DI. Incorporation of soya oil hydrolysate in the diet of defaunated or refaunated sheep: effect on rumen fermentation in vitro. Arch Anim Nutr 1990, 40: 329-337.
  44. Broudiscou L, Van Nevel CJ, Demeyer DI. Effect of soya oil hydrolysate on rumen digestion in defaunated and refaunated sheep. Anim Feed Sci Technol 1990, 30: 51-67.
  45. Binelli M, Vanderkooi MK, Chapin LT, Vandehaar MJ, Turner JD, Moseley WM, Tucker HA. Comparison of growth hormone-releasing factor and somatotropin: Body growth and lactation of primiparous cows. J Dairy Sci 1995, 78: 2129-2139.
  46. Kandler O, König H. Cell envelopes of archaebacteria. In: Woese CR, Wolfe RS (Eds), The Bacteria, Volume VIII, Archaebacteria. Academic Press Inc., London, 1985. p 413-457.
  47. Henderson C. The effects of fatty acids on pure cultures of rumen bacteria. J Agric Sci (Camb) 1973, 81: 107-112.
  48. Williams AG, Coleman GS. The Rumen Protozoa. Springer-Verlag, New York. 1992.
  49. Tokura M, Ushida K, Miyazaki K, Kojima Y. Methanogens associated with rumen ciliates. FEMS Microbiol Ecol 1997, 22: 137-143.
  50. Hobson PN, Stewart CS. The Rumen Microbial Ecosystem. Chapman & Hall, London. 1997.


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