Free Access
Reprod. Nutr. Dev.
Volume 43, Number 4, July-August 2003
Page(s) 347 - 356
Reprod. Nutr. Dev. 43 (2003) 347-356
DOI: 10.1051/rnd:2003028

Fructooligosaccharide associated with celecoxib reduces the number of aberrant crypt foci in the colon of rats

Bruno Buechera, Cécile Thouminota, Jean Menanteaub, Christian Bonnetc, Anne Jarrya, Marie-Françoise Heymanna, Christine Cherbutc, Jean-Paul Galmichea and Hervé M. Blottièrea, c, d

a  Centre de Recherche en Nutrition Humaine de Nantes, INSERM U539, CHU Hôtel-Dieu, 44035 Nantes, France
b  Centre de Recherche en Nutrition Humaine de Nantes, INSERM U419, CHU Hôtel-Dieu, 44035 Nantes, France
c  Centre de Recherche en Nutrition Humaine de Nantes, INRA-UFDNH, CHU Hôtel-Dieu, 44035 Nantes, France
d  Present address: UNSA-INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France

(Received 24 March 2003; accepted 24 June 2003)

According to Burkitt's hypothesis, dietary fibres may protect against the development of colorectal cancer. In rats, studies have shown that only butyrate-producing fibres are protective. In parallel, in humans, non-steroidal anti-inflammatory drugs, which target cyclooxygenases, have been shown to display a protective effect against colorectal cancer. Among them, COX-2-selective inhibitors which present less side effects than non-selective agents, are promising as chemopreventive agents. Our aim was to analyse the effect of an association between butyrate-producing fibres and the COX-2 inhibitor on the development of aberrant crypt foci (ACF) in rats. Fisher F344 rats were fed with (1) a standard low fibre control diet; (2) the standard diet supplemented with 1500 ppm celecoxib; (3) a diet supplemented with 6% fructo-oligosaccharide (FOS); and (4) a diet with both celecoxib and FOS. Three weeks later, the rats were injected twice with azoxymethane and the number of ACF was determined 15 weeks later. In the control group, $43.8 \pm 6.4$ ACF were found. This number was not significantly modified by the addition of FOS or celecoxib alone to the diet. However, the association of FOS and celecoxib resulted in a 61% reduction in the number of ACF ( P < 0.01). The number of aberrant crypt per foci was also reduced. Thus, although no significant effect of celecoxib or FOS alone was identified, the association of butyrate-producing fibre and celecoxib was effective in preventing the development of ACF. This preliminary study argues for a strong protective effect of such an association which deserves further studies.

Key words: COX-2 / butyrate / colorectal cancer / NSAID

Correspondence and reprints: Hervé M. Blottière

© INRA, EDP Sciences 2003