Reprod. Nutr. Dev.
Volume 43, Number 4, July-August 2003
|Page(s)||325 - 330|
Cryopreservation of bovine oocytes: Current status and recent developmentsAlban Massip
Université catholique de Louvain, Institut des Sciences de la Vie, Unité des Sciences vétérinaires, Place Croix du Sud 5 Bte 10, 1348 Louvain-la-Neuve, Belgium
(Received 22 November 2002; accepted 17 June 2003)
The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 °C. Therefore, cryopreservation of oocytes by vitrification - especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications - seems the most appropriate method.
Key words: oocyte / bovine / cryopreservation
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© INRA, EDP Sciences 2003