Free Access
Reprod. Nutr. Dev.
Volume 46, Number 5, September-October 2006
5th annual INRA-Meeting: Mammary gland and milk
Page(s) 557 - 566
Published online 23 September 2006
Reprod. Nutr. Dev. 46 (2006) 557-566
DOI: 10.1051/rnd:2006027

Sulphated proteins secreted by rat mammary epithelial cells

Eric Chanat

Institut National de la Recherche Agronomique, Laboratoire de Génomique et Physiologie de la Lactation, Jouy-en-Josas, 78352, France

(Published online 23 September 2006)

Abstract - The main sulphated proteins secreted by rat mammary gland tissue have Mr of approximately 32 000, 27 000 and 25 000 Da. In addition, there are high Mr components which have a diffuse electrophoretic mobility (Mr > 200 000) and most likely corresponded to proteoglycans. The sulphate groups in the proteins with discrete Mr are most likely all linked to carbohydrates. These sulphated molecules were partially purified and identified to isoforms of rat $\alpha $-lactalbumin for the 25-27 kDa bands and to $\kappa $-casein for the 32 kDa band. This pattern of protein sulphation is, as far as we know, quite specific to rat mammary epithelial cells.

Key words: mammary gland / epithelial cell / milk protein / caseins / alpha-lactalbumin / sulphation

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© INRA, EDP Sciences 2006