Open Access
Reprod. Nutr. Dev.
Volume 46, Number 2, March-April 2006
Page(s) 211 - 218
Published online 06 April 2006
Reprod. Nutr. Dev. 46 (2006) 211-218
DOI: 10.1051/rnd:2006005

Production of butter fat rich in trans10-C18:1 for use in biomedical studies in rodents

Alexandre Roy, Anne Ferlay and Yves Chilliard

Herbivore Research Unit INRA-Theix, 63122 St-Genès-Champanelle, France

(Received 22 August 2005; accepted 3 November 2005; published online 6 April 2006)

Abstract - Trans fatty acids are suspected to be detrimental to health, particularly to cardiovascular function. Trans fatty acids include a wide range of fatty acids, with isomers of C18:1, conjugated and non-conjugated C18:2 as major components. A vaccenic acid (trans11-C18:1) + rumenic acid (cis9,trans11-CLA)-rich butter has been shown previously to exhibit health beneficial effects, but less is known concerning another trans-C18:1 present in hydrogenated vegetable oil-based products and sometimes in milk fat, the trans10-isomer. The present experiment was conducted to produce butters from milk of variable fatty acid composition for use in biomedical studies with rodents, with the overall aim of evaluating the specific effect of trans10-C18:1 and trans11-C18:1 + cis9,trans11-CLA on cardiovascular function. Milks from lactating dairy cows fed two types of maize-based diets supplemented (5% of dry matter) - or not - with sunflower oil were collected, and used to manufacture butters either rich in trans10-C18:1 (14% of total fatty acids, 64.5% of fat content) or rich in trans11-C18:1 + cis9,trans11-CLA (7.4 and 3.1% of total fatty acids, respectively, 68.5% of fat content), or with standard fatty acid composition (70% of fat content). Additionally, total saturated fatty acid percentage was reduced by more than one third in the enriched butters compared with the standard butter. An understanding of the role of nutrition on milk fatty acid composition in cows allows for the production of dairy products of variable lipid content and composition for use in biomedical studies in animal models and human subjects.

Key words: trans10-C18:1 / trans11-C18:1 / conjugated linoleic acid / butter

Corresponding author: Yves Chilliard

© INRA, EDP Sciences 2006