Reprod. Nutr. Dev.
Volume 46, Number 1, January-February 2006
|Page(s)||97 - 104|
|Published online||27 January 2006|
Nuclear and cytoplasmic localization of interferon- in in vitro-produced bovine blastocystsKelly M. Johnsona, Xavier Alvarezb, Olga N. Borkhseniousc and H. Michael Kubischa
a Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA 70433, USA
b Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA
c School of Veterinary Medicine, Louisiana State University, Baton Rouge LA 70803, USA
(Received 21 March 2005; accepted 18 April 2005; published online 27 January 2006)
Abstract - Experiments were conducted to detect interferon- in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN- in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN- in the trophectoderm of blastocysts. The distribution of IFN- appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.
Key words: interferon- / embryo / blastocyst / gene expression / IVF
Corresponding author: H. Michael Kubisch mkubisch@tul
© INRA, EDP Sciences 2006