Nuclear and cytoplasmic localization of interferon-$\tau$ in in vitro-produced bovine blastocysts

Kelly M. Johnson; Xavier Alvarez; Olga N. Borkhsenious; H. Michael Kubisch

doi:doi:10.1051/rnd:2005060

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Volume 46 / No 1 (January-February 2006)

Reprod. Nutr. Dev., 46 1 (2006) 97-104

Abstract

Free Access

Issue		Reprod. Nutr. Dev. Volume 46, Number 1, January-February 2006


Page(s)		97 - 104
DOI		https://doi.org/10.1051/rnd:2005060
Published online		27 January 2006

Reprod. Nutr. Dev. 46 (2006) 97-104
DOI: 10.1051/rnd:2005060

Nuclear and cytoplasmic localization of interferon- $\tau$ in in vitro-produced bovine blastocysts

Kelly M. Johnson^a, Xavier Alvarez^b, Olga N. Borkhsenious^c and H. Michael Kubisch^a

^a Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA 70433, USA
^b Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA
^c School of Veterinary Medicine, Louisiana State University, Baton Rouge LA 70803, USA

(Received 21 March 2005; accepted 18 April 2005; published online 27 January 2006)

Abstract - Experiments were conducted to detect interferon- $\tau$ in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN- $\tau$ in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN- $\tau$ in the trophectoderm of blastocysts. The distribution of IFN- $\tau$ appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.

Key words: interferon- $\tau$ / embryo / blastocyst / gene expression / IVF

Corresponding author: H. Michael Kubisch mkubisch@tul

© INRA, EDP Sciences 2006

Nuclear and cytoplasmic localization of interferon- in in vitro-produced bovine blastocysts

Nuclear and cytoplasmic localization of interferon- $\tau$ in in vitro-produced bovine blastocysts