Free Access
Reprod. Nutr. Dev.
Volume 45, Number 3, May-June 2005
SYMPOSIUM: Homage to Professor Charles Thibault
Page(s) 255 - 259
Reprod. Nutr. Dev. 45 (2005) 255-259
DOI: 10.1051/rnd:2005018

Involvement of equine chorionic gonadotropin (eCG) carbohydrate side chains in its bioactivity; lessons from recombinant hormone expressed in insect cells

Sébastien Legardinier, Claire Cahoreau, Danièle Klett and Yves Combarnous

Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS) et Université François Rabelais de Tours, 37 380 Nouzilly, France

Abstract - Natural eCG consists of as much as 45% carbohydrate side chains. The present paper deals with the analysis of the roles of the N- and O-linked saccharides of this hormone in the different steps of its activity and its possible repacement by recombinant eCG expressed in baculovirus - insect cell systems.

Key words: half-life / gonadotropin / carbohydrate / recombinant hormone / baculovirus

Corresponding author: Yves Combarnous

© INRA, EDP Sciences 2005