Issue |
Reprod. Nutr. Dev.
Volume 44, Number 2, March-April 2004
|
|
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Page(s) | 131 - 140 | |
DOI | https://doi.org/10.1051/rnd:2004020 |
DOI: 10.1051/rnd:2004020
Myristic acid increases
6-desaturase activity
in cultured rat hepatocytes
Sophie Jan, Hervé Guillou, Sabine D'Andrea, Stéphanie Daval, Monique Bouriel, Vincent Rioux and Philippe Legrand Laboratoire de Biochimie, INRA-ENSA, 65 rue de Saint-Brieuc, CS84215, 35042 Rennes, France
(Received 5 January 2004; accepted 26 January 2004)
Abstract - In order to study the effects of saturated fatty acids on
6-desaturase activity, rat hepatocytes in primary culture were incubated with lauric (C12:0), myristic (C14:0) or palmitic
(C16:0) acids. After optimization, the standard in vitro conditions for the measurement of
6-desaturase activity were as follows: 60
mol·L-1
-linolenic acid (C18:3n-3), reaction time of 20 min and protein content of 0.4 mg. Data showed that cell treatment with 0.5
mmol·L-1 myristic acid during 43 h specifically increased
6-desaturase activity. This improvement, reproducible for three substrates of
6-desaturase, i.e. oleic acid (C18:1n-9), linoleic acid (C18:2n-6) and
-linoleic acid (C18:3n-3) was dose-dependent in the range 0.1-0.5 mmol·L-1 myristic acid concentration.
Key words: myristic acid /

Corresponding author: Philippe Legrand philippe.legrand@agrocampus-rennes.fr
© INRA, EDP Sciences 2004