Free Access
Reprod. Nutr. Dev.
Volume 44, Number 2, March-April 2004
Page(s) 105 - 109
Reprod. Nutr. Dev. 44 (2004) 105-109
DOI: 10.1051/rnd:2004018

In vitro maturation rates of canine oocytes from anoestrous bitches in simple media

Ada Rota and Giorgia Cabianca

Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Padua, Italy

(Received 13 November 2003; accepted 8 January 2004)

Abstract - The meiotic competence of canine oocytes collected from anoestrous bitch ovaries and cultured for 72 h in different media was studied. The base culture medium was TCM 199 enriched with 10% fetal bovine serum (TCM); the effect of supplementation with EGF (50 ng·mL-1) or ITS (insulin: 10 $\mu$g·mL-1; transferrin: 5.5 $\mu$g·mL-1; selenium: 5 $\mu$g·mL-1) was also studied. TCM was also compared to a Synthetic Oviductal Fluid (SOF). All the media contained FSH (0.1 UI·mL-1), LH (10 UI·mL-1), 17 $\beta$-oestradiol (4 $\mu$g·mL-1) and kanamycin. Despite the anoestrous stage of the donor bitches, resumption of meiosis occurred in a high proportion of the oocytes, (mean value 77.3%). The number of oocytes showing the 'germinal vesicle breakdown' nuclear stage was not influenced by the type of the culture medium used. ITS had a positive effect on nuclear progression to later stages (from metaphase I to metaphase II); however, this effect was not statistically significant.

Key words: bitch / anoestrous / oocytes / maturation / in vitro

Corresponding author: Ada Rota

© INRA, EDP Sciences 2004