Free Access
Issue |
Reprod. Nutr. Dev.
Volume 41, Number 6, November-December 2001
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Page(s) | 465 - 485 | |
DOI | https://doi.org/10.1051/rnd:2001102 |
DOI: 10.1051/rnd:2001102
Reprod. Nutr. Dev. 41 (2001) 465-485
School of Science and Engineering, University of Abertay Dundee, Kydd Building, Bell Street, Dundee DD1 1HG, UK
Key words: transgenesis / gene targeting / gene therapy / integration / homologous recombination / concatenation
Correspondence and reprints: Kevin Smith
e-mail: k.smith@abertay.ac.uk
© INRA, EDP Sciences 2001
Reprod. Nutr. Dev. 41 (2001) 465-485
Theoretical mechanisms in targeted and random integration of transgene DNA
Kevin SmithSchool of Science and Engineering, University of Abertay Dundee, Kydd Building, Bell Street, Dundee DD1 1HG, UK
(Received 4 September 2001; accepted 10 December 2001)
Abstract
The genetic manipulation of mammalian cells and animals would be greatly expedited
if gene targeting could be reliably achieved in the widest possible range of host
cell types. This paper considers empirical evidence and theoretical considerations
associated with transgene integration, and concludes that utilisation of gene
targeting in non-selective systems awaits further progress in modelling homologous
recombination.
Key words: transgenesis / gene targeting / gene therapy / integration / homologous recombination / concatenation
Correspondence and reprints: Kevin Smith
e-mail: k.smith@abertay.ac.uk
© INRA, EDP Sciences 2001