Issue |
Reprod. Nutr. Dev.
Volume 42, Number 1, January-February 2002
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|
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Page(s) | 73 - 80 | |
DOI | https://doi.org/10.1051/rnd:20020008 |
Reprod. Nutr. Dev. 42 (2002) 73-80
DOI: 10.1051/rnd:20020008
Cryopreservation of bovine oocytes: is cryoloop vitrification the future to preserving the female gamete?
Andreas Mavridesa and David Morrollba Department of Human Development, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK
b NURTURE, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK
(Received 24 October 2001; accepted 30 January 2002)
Abstract
The cryoloop is a technique where a thin nylon loop is used to suspend a film of
cryoprotectant containing the oocytes and directly immersing them in liquid nitrogen. 508
bovine oocytes were collected, of these 351 were cryopreserved by slow freezing using
standard straws or a new vitrification method using our self-constructed cryoloops and the
remainder were controls. After thawing, the oocytes were inseminated by ICSI or standard IVF.
The cryoloop vitrification method yielded a survival rate of 90.5% and the slow freezing
technique a rate of 54.4% (
p < 0.0001). When ICSI was performed, cryopreservation by the
cryoloop vitrification method resulted in very similar cleavage rate to controls (16.0% vs.
17.3%) but slow freezing produced a slightly lower rate (9.4%). Cleavage rates after IVF in
fresh oocytes was higher than the cryopreservation groups (49.5% vs. 15.4% and 25.8%),
whereas after ICSI the rates were similar in all groups (17.3% vs. 9.4% and 16%). It is
concluded that the new cryoloop vitrification technique followed by ICSI produce good embryo
formation results and they could hold the future for effective oocyte cryopreservation.
Key words: bovine / cryoloop / ICSI / oocyte / vitrification
Correspondence and reprints: Andreas Mavrides andreas@mavrides.com
© INRA, EDP Sciences 2002