Myristic acid increases 6-desaturase activity in cultured rat hepatocytesSophie Jan, Hervé Guillou, Sabine D'Andrea, Stéphanie Daval, Monique Bouriel, Vincent Rioux and Philippe Legrand
Laboratoire de Biochimie, INRA-ENSA, 65 rue de Saint-Brieuc, CS84215, 35042 Rennes, France
(Received 5 January 2004; accepted 26 January 2004)
Abstract - In order to study the effects of saturated fatty acids on 6-desaturase activity, rat hepatocytes in primary culture were incubated with lauric (C12:0), myristic (C14:0) or palmitic (C16:0) acids. After optimization, the standard in vitro conditions for the measurement of 6-desaturase activity were as follows: 60 mol·L-1 -linolenic acid (C18:3n-3), reaction time of 20 min and protein content of 0.4 mg. Data showed that cell treatment with 0.5 mmol·L-1 myristic acid during 43 h specifically increased 6-desaturase activity. This improvement, reproducible for three substrates of 6-desaturase, i.e. oleic acid (C18:1n-9), linoleic acid (C18:2n-6) and -linoleic acid (C18:3n-3) was dose-dependent in the range 0.1-0.5 mmol·L-1 myristic acid concentration.
Key words: myristic acid / 6-desaturase / saturated fatty acids / cultured rat hepatocytes
Corresponding author: Philippe Legrand firstname.lastname@example.org
© INRA, EDP Sciences 2004