Free access
Issue
Reprod. Nutr. Dev.
Volume 44, Number 5, September-October 2004
Page(s) 419 - 435
DOI http://dx.doi.org/10.1051/rnd:2004049
Reprod. Nutr. Dev. 44 (2004) 419-435
DOI: 10.1051/rnd:2004049

Estimation of ileal output of gastro-intestinal glycoprotein in weaned piglets using three different methods

Christelle Piel, Lucile Montagne, Paulo Salgado and Jean-Paul Lallès

Unité Mixte (INRA-Agrocampus Rennes) de Recherche sur le Veau et le Porc, Domaine de la Prise, 35590 Saint-Gilles, France

(Received 19 February 2004; accepted 26 June 2004)

Abstract - Mucin is the main constituent of gastrointestinal mucus and is responsible for its physico-chemical and physiological properties. Previous studies have suggested that this glycoprotein represents a major component of undigested endogenous protein at the ileum. The aim of the study was to estimate the ileal output of this glycoprotein using three methods: direct ELISA, hexosamine-based method and ethanol precipitation. For setting up the ELISA assay, the glycoprotein was isolated from intestinal mucus scraping by cesium chloride density gradient ultracentrifugation and a rabbit hyperimmune plasma was raised against the purified glycoprotein. Ileal outputs of hexosamine and glycoprotein were measured in weaned piglets fed a control diet (C) based on casein or diets which contained 50% crude protein supplied by white (WCP) or black (BCP) chickpea. The hexosamine output was higher (P < 0.05) with the WCP diet (2.3 and 1.5 g·kg-1 of dry matter intake for glucosamine and galactosamine, respectively) than with diet C (1.1 and 0.7 g·kg-1 of DMI). The hexosamine-based and ethanol precipitation methods, but not the ELISA, showed significant differences between the diet treatments (P < 0.05). Although hexosamine-based and ethanol precipitation methods for the estimation of ileal glycoprotein appeared to be more satisfactory than the developed ELISA to display diet effects in this study, it remains to be determined whether the higher glycoprotein data variability observed with ELISA reflects the actual biological variability of the phenomenon or not.


Key words: small intestine / methods / glycoprotein / piglet

Corresponding author: Jean-Paul Lallès Jean-Paul.Lalles@rennes.inra.fr

© INRA, EDP Sciences 2004

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access.

An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

  • If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
  • You can define your own OpenURL resolver with your EDPS Account.
    In this case your choice will be given priority over that of your library.
  • You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.